Current topics in cellular regulation Vol. 4 by Bernard L. Horecker, Earl R. Stadtman
By Bernard L. Horecker, Earl R. Stadtman
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Study of Repressore Altered by Mutations 61 VI. The Operator 63 A. Assay and Purification 63 B. Size and Properties 63 VII. Positive Control of the Lac Operon 65 VIII. The Mode of Action of the Lac Repressor 67 IX. Generalization 68 References 72 I. Introduction The lactose (lac) operon of Escherichia coli, its products and its regulation have been the subject of many reviews and of a recent comprehensive book (5). These have covered the extensive background of knowledge gathered over 25 years of in vivo studies mostly by Jacob and Monod and their co-workers and which led them to formulate in 1961 (36) their model of negative control of protein synthesis.