ANSI Common Lisp - Solutions by Paul Graham.

By Paul Graham.

ANSI universal Lisp recommendations.

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8 with KOH. 2. Liquid MS medium: Similar to MS-agar plate medium but without plant agar. 3. 15 mM Filipin stock solution (10 mg/ml): 1 mg of filipin III from Streptomyces filipinensis dissolved in 100 μl of dimethyl sulfoxide (DMSO). Be sure filipin is dissolved completely, including particles in the cap of the vial. Filipin is sensitive to freezing/thawing; resuspension of filipin is done at room temperature since resuspension on ice would lead to DMSO freezing. Filipin solution is aliquoted and can be stored in the dark at −20 °C for 2 weeks maximum.

Eur J Biochem 207: 125–134 Chapter 3 Live Microscopy Analysis of Endosomes and Vesicles in Tip-Growing Root Hairs Miroslav Ovečka, Irene Lichtscheidl, and Jozef Šamaj Abstract Tip growth is one of the most preferable models in the study of plant cell polarity; cell wall deposition is restricted mainly to a certain area of the cell, and cell expansion at this specific area leads to the development of tubular outgrowth. Tip-growing root hairs are well-established systems for such studies, because their lateral position within the root makes them easily accessible for experimental approaches and microscopic observations.

3. For documentation of endosomes with UV microscopy, mount plants in the quartz slides. Transfer them to the UV microscope. Set appropriate wavelength (generally within the range of 280– 360 nm) by the monochromator, and acquire images using UV-sensitive camera. Record the root hairs for short periods of time, to prevent harmful effects of the UV light (see Note 17). 4. The video computer offers tracking functions for the analysis of endosomal movements. Every second, third, or fourth frame can be displayed on the monitor screen, thus forming an integrated picture.

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